Diafiltración

La diafiltración es un proceso de dilución que implica la eliminación o separación de componentes (moléculas permeables como sales, pequeñas proteínas, disolventes, etc.) de una solución en función de su tamaño molecular mediante el uso de filtros permeables de micromoléculas para obtener una solución pura.^[1]^[2]^[3]^[4]^[5]^[6]^[7]

Referencias[editar]

↑ Shao, Jiahui; Zydney, Andrew L. (2004). «Optimization of Ultrafiltration/Diafiltration Processes for Partially Bound Impurities». Biotechnology and Bioengineering 87 (3): 286-292. PMID 15281103. doi:10.1002/bit.20113.
↑ Blatt, W.F.; Robinson, S.M.; Bixler, Harris J. (Oct 1968). «Membrane ultrafiltration: The diafiltration technique and its application to microsolute exchange and binding phenomena». Analytical Biochemistry 26 (1): 151-173. PMID 5761168. doi:10.1016/0003-2697(68)90039-0.
↑ Schwartz, Larry. «Diafiltration: A Fast, Efficient Method for Desalting, or Buffer Exchange of Biological Samples». Literature Library.
↑ «Protein Concentration and Diafiltration by Tangential Flow Filtration». Millipore.
↑ Sweeney, Scott F.; Woehrle, Gerd H.; Hutchison, James E. (2006). «Rapid Purification and Size Separation of Gold Nanoparticles via Diafiltration». Journal of the American Chemical Society 128 (10): 3190-3197. PMID 16522099. doi:10.1021/ja0558241.
↑ Schwartz, Larry. «Diafiltration for Desalting or Buffer Exchange». BioProcess International.
↑ «Diafiltration (Buffer Exchange) Using Hollow Fiber Membranes instead of Dialysis Tubing - Automated Diafiltration». Archivado desde el original el 19 de junio de 2018. Consultado el 20 de septiembre de 2020.

Otras lecturas[editar]

Bowen, W. Richard; Mohammad, A. Wahab (Aug 1998). «Diafiltration by nanofiltration: Prediction and optimization». AIChE Journal 44 (8): 1799-1812. doi:10.1002/aic.690440811.
Limayem, Imène; Charcosset, Catherine; Fessi, Hatem (2004). «Purification of nanoparticle suspensions by a concentration/diafiltration process». Separation and Purification Technology 38 (1): 1-9. doi:10.1016/j.seppur.2003.10.002.
Sheth, Jignesh P. (2003). «Nanofiltration-based diafiltration process for solvent exchange in pharmaceutical manufacturing». Journal of Membrane Science 211 (2): 251-261. doi:10.1016/s0376-7388(02)00423-4.

Enlaces externos[editar]

Datos: Q1208096

[1] Shao, Jiahui; Zydney, Andrew L. (2004). «Optimization of Ultrafiltration/Diafiltration Processes for Partially Bound Impurities». Biotechnology and Bioengineering 87 (3): 286-292. PMID 15281103. doi:10.1002/bit.20113.

[2] Blatt, W.F.; Robinson, S.M.; Bixler, Harris J. (Oct 1968). «Membrane ultrafiltration: The diafiltration technique and its application to microsolute exchange and binding phenomena». Analytical Biochemistry 26 (1): 151-173. PMID 5761168. doi:10.1016/0003-2697(68)90039-0.

[3] Schwartz, Larry. «Diafiltration: A Fast, Efficient Method for Desalting, or Buffer Exchange of Biological Samples». Literature Library.

[4] «Protein Concentration and Diafiltration by Tangential Flow Filtration». Millipore.

[5] Sweeney, Scott F.; Woehrle, Gerd H.; Hutchison, James E. (2006). «Rapid Purification and Size Separation of Gold Nanoparticles via Diafiltration». Journal of the American Chemical Society 128 (10): 3190-3197. PMID 16522099. doi:10.1021/ja0558241.

[6] Schwartz, Larry. «Diafiltration for Desalting or Buffer Exchange». BioProcess International.

[7] «Diafiltration (Buffer Exchange) Using Hollow Fiber Membranes instead of Dialysis Tubing - Automated Diafiltration». Archivado desde el original el 19 de junio de 2018. Consultado el 20 de septiembre de 2020.

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